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        信號通路磷酸化廣篩抗體芯片(PEX100)
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        信號通路磷酸化廣篩抗體芯片(PEX100)

        華盈生物PEX100磷酸化廣譜篩選抗體芯片可同時對30多條信號通路磷酸化和蛋白表達水平進行同步篩選,是解決信號通路研究復雜性的有利工具
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              從細胞膜、胞漿到細胞核,存在多條信號通路串聯交叉形成的復雜信號網絡。該信號網絡在細胞受到胞外刺激后將信號通過級聯放大、分散調節等方式傳入胞內,引起一系列的綜合性細胞應答。一種生物效應的出現往往存在多條信號通路的同步活化,可逆的磷酸化修飾反應則是細胞內部最為普遍和節能的信號蛋白活化調節方式。因此,找到激活的信號通路乃至發生磷酸化調變的通路蛋白,往往成生命科學研究的起點。

         

         

         
         
         

              信號通路磷酸化廣譜篩選抗體芯片(PEX100),采用三維高分子膜專利技術,在片基上高密度結合  1318  種高特異抗體,分別檢測432個信號蛋白的679個磷酸化位點。這些信號蛋白廣泛參與多條重要信號通路信號傳導過程。芯片針對每一個特定蛋白磷酸化位點,設置一對抗體分別檢測其磷酸化(Phospho)和非磷酸化(non-Phospho)狀態以提高磷酸化檢測靈敏度和穩定性。一次芯片實驗即可實現多條信號通路的同步篩選和具體調變位點的清晰定位,為后續生物現象的深入探索提供明確的研究方向。

        |   抗體芯片特點

        ●   芯片規格為76 x 25 x 1 mm;

        ●   實現31條信號通路全面篩選;

        ●   每種抗體設置2次技術重復;

        ●   適用于組織、細胞等多類型樣本;

        ●   5 x106細胞、100μg總蛋白量即可滿足實驗;

        ●   每個檢測位點設有磷酸化和非磷酸化配對抗體;

        ●   可通用于人、小鼠、大鼠等多類型模式生物檢測。

         

        |   芯片檢測范圍

        信號通路

        蛋白數

        信號通路

        蛋白數

        MAPK signaling pathway

        67

        Interleukin signaling pathway 37

        Erk signaling pathway

        59

        Regulation of actin cytoskeleton

        36

        Angiogenesis

        57

        Jak-STAT signaling pathway

        32

        Focal adhesion

        56

        AKT/PKB signaling pathway

        31

        Neurotrophin signling pathway

        52

        FGF signaling pathway

        29

        ErbB signaling pathway

        45

        Endocytosis

        29

        AKT signaling pathway

        45

        Integrin signaling pathway

        29

        Chemokine signaling pathway

        45

        VEGF signaling pathway

        27

        PDGF signaling pathway

        43

        Wnt signaling pathway

        26

        T cell receptor signaling pathway

        43

        p53 signaling pathway

        25

        Apoptosis signaling pathway

        41

        Adipocytokine signaling pathway

        19

        EGF receptor signaling pathway

        41

        Calcium signaling pathway

        19

        Insulin signaling pathway

        39

        Insulin/IGF pathway

        16

        CREB signaling pathway

        39

        mTOR signaling pathway

        16

        Cell cycle

        37

        TGF-beta signaling pathway

        13

         

        |   抗體芯片原理

         

        |   客戶案例

         

        PEX100芯片協助揭秘衰老引起的營養不良潛在機制

         

         

              研究人員采集了不同月齡的正常B6小鼠的小腸進行組織學觀察尋找到衰老引起營養吸收障礙的靶器官主要在空腸絨毛隱窩區域。經文獻調研及實驗研究發現,mTORC1蛋白在老齡小鼠腸絨毛老化過程中發揮著關鍵作用,mTORC1通路是如何調控腸絨毛老化的呢?研究人員果斷采用PEX100磷酸化廣篩抗體芯片技術,對Villin-Cre;Tsc1f/f小鼠的小腸組織進行廣泛的蛋白磷酸化篩選??贵w芯片迅速鎖定了mTORC1下游一系列關鍵分子,其中p38MAPK蛋白的在Villin-Cre;Tsc1f/f小鼠中上調7.21倍,尤為顯著。

              研究人員利用Westernblot證實了p38MAPK以及其上游的MKK6蛋白在Tsc1−/−腸絨毛中顯著上調,而雷帕霉素的施用可以逆轉該現象。為進一步證實MKK6與p38MAPK的調控關系,在體外培養的MEFs細胞中,利用順轉siRNA的方式敲降MKK6后,發現p38MAPK的磷酸化變化受到了MKK6的調控,即說明MKK6是介于mTORC1和p38MAPK的中間信號轉導蛋白。

              研究人員通過一系列的研究,逐步明確了mTORC1-MKK6-p38MAPK-p53通路在腸絨毛老化中發揮著關鍵作用,并為逆轉腸絨毛老化,改善衰老引起的營養不良找到了潛在的治療靶點和候選藥物小分子。

         

        詳細內容點擊查看https://mp.weixin.qq.com/s/_tG9Y9v-5oh3qPDvAtPGjw

        |   客戶文獻

        [1]. Fu Liwei, Li Pinxue, Zhu Junyao et al. Tetrahedral framework nucleic acids promote the biological functions and related mechanism of synovium-derived mesenchymal stem cells and show improved articular cartilage regeneration activity in situ. Bioact Mater, 2022, 9: 411-427.  IF=14.593

        [2]. Geng YJ, Chen SL, Yang Y, et al. Long-term exposure to genistein inhibits the proliferation of gallbladder cancer by downregulating the MCM complex. Science Bulletin, 2022(1). 2022 Apr il; 67(8): p. 813-824. IF=11.780

        [3]. Du Changhong, Wang Xinmiao, Wu Yiding, et al. Renal Klotho and inorganic phosphate are extrinsic factors that antagonistically regulate hematopoietic stem cell maintenance. Cell Rep, 2022, 38: 110392.  IF=9.423

        [4]. Lin Yao-Xin, Wang Yi, Ding Jianxun, et al. Reactivation of the tumor suppressor PTEN by mRNA nanoparticles enhances antitumor immunity in preclinical models. Sci Transl Med, 2021, 13: undefined.  IF=17.956

        [5]. Jiang Wenhao, Jin Yunyun, Zhang Shiwei, et al. PGE2 activates EP4 in subchondral bone osteoclasts to regulate osteoarthritis.[J] .Bone Res, 2022, 10: 27.  IF=13.567

        [6]. Zhu Rongjia, Yan Tingdong, Feng Yingmei, et al. Mesenchymal stem cell treatment improves outcome of COVID-19 patients via multiple immunomodulatory mechanisms. Cell Res, 2021, 31: 1244-1262.  IF=25.617

        [7]. Li Hui, Jin Yuhong, Zhao Yu, et al. Targeted cell therapy for partial-thickness cartilage defects using membrane modified mesenchymal stem cells by transglutaminase 2. Biomaterials, 2021, 275: 120994.  IF=12.479

        [8]. Long Hui, Yu Weina, Yu Sha, et al. Progesterone affects clinic oocyte yields by coordinating with follicle stimulating hormone via PI3K/AKT and MAPK pathways. J Adv Res, 2021, 33: 189-199.  IF=10.479

        [9]. Huang Tinglei, Chen Biying, Wang Feng, et al. Rab1A promotes IL-4R/JAK1/STAT6-dependent metastasis and determines JAK1 inhibitor sensitivity in non-small cell lung cancer. Cancer Lett, 2021, 523: 182-194.  IF=8.679

        [10]. Zhao Qun, Guo Jian, Wang Guizhen, et al. CXCL13 promotes intestinal tumorigenesis through the activation of epithelial AKT signaling. Cancer Lett, 2021, 511: 1-14.  IF=8.679

        [11]. He D, Wu HG, Xiang JN, et al. Gut stem cell aging is driven by mTORC1 via a p38 MAPK-p53 pathway. Nat Commun. 2020 Jan 2;11(1):37.   IF=14.919

        [12]. Chen WL, Jin X,Wang MS, et al. GLUT5-mediated fructose utilization drives lung cancer growth by stimulating fatty acid synthesis and AMPK/mTORC1 signaling. JCI Insight. 2020 Feb 13;5(3):e131596.   IF=8.315

        [13]. Yan LX, Li HY, Xia WL. Bioglass could increase cell membrane fluidity with ion products to develop its bioactivity. Cell Prolif. 2020 Nov;53(11):e12906.   IF=6.831

        [14]. Gan XJ, Zhang RS, Gu J, et al. Acidic Microenvironment Regulates the Severity of Hepatic Ischemia/Reperfusion Injury by Modulating the Generation and Function of Tregs via the PI3K-mTOR Pathway. Front Immunol. 2020 Jan 9;10:2945.  IF=7.561

        [15]. He D, Wu HG, Xiang JN, et al. Gut stem cell aging is driven by mTORC1 via a p38 MAPK-p53 pathway. Nat Commun. 2020 Jan 2;11(1):37.   IF=14.919

        [16]. Zeng Q, Liu YM, Liu J, et al. Inhibition of ZIP4 reverses epithelial-to-mesenchymal transition and enhances the radiosensitivity in human nasopharyngeal carcinoma cells. Cell Death Dis. 2019 Aug 5;10(8):588.  IF=8.469

        [17]. Yin LM, Xu YD, Peng LL, et al. Transgelin-2 as a therapeutic target for asthmatic pulmonary resistance. Sci Transl Med. 2018 Feb 7;10(427):eaam8604.   IF=17.956

        [18]. Gao YY, Bai XF, Zhang DJ , et al. Mammalian elongation factor 4 regulates mitochondrial translation essential for spermatogenesis. Nat Struct Mol Biol. 2016 May;23(5):441-9.   IF=15.369

        [19]. Jiang HL, Sun HF, Gao SP, et al. SSRP1 uppresses TGF-β-Driven Epithelial-to-Mesenchymal Transition and Metastasis in Triple-Negative Breast Cancer by Regulating Mitochondrial Retrograde Signaling, Cancer Res. 2016 Feb 15;76(4):952-64.   IF=12.701

        [20]. Kuang XY, Jiang HS, Li K, et al. The phosphorylation-specific association of STMN1 with GRP78 promotes breast cancer metastasis. Cancer Lett. 2016 Jul 10;377(1):87-96.   IF=8.679

        [21]. Jia DS, Jing Y, Zhang ZF, et al. Amplification of MPZL1/PZR promotes tumor cell migration through Src-mediated phosphorylation of cortactin in hepatocellular carcinoma. Cell Res. 2014 Feb;24(2):204  IF=25.617

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        重生之玩遍女明星目录,庙中求子被僧人C燕氏,辣文小寡妇,性奴大乳清纯校花调教记